COMPARISON OF DIFFERENT METHODS FOR BETA LACTOGLOBULIN ISOLATION

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dc.contributor.authorEzgi Demir Özer
dc.contributor.authorZübeyde Öner
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractThe aim of this study was to introduce a simple, high efficient and less expensive method for isolation of ?Lg from whey. Anion exchange chromatography, pepsin enzyme treatment and ultrafiltration tecniques were preferred for isolation process to compare differences. In addition to, centrifuge ultrafiltration techniques were using for the first time for isolation of ?-Lg from whey. Physicochemical analysis of whey samples indicated that protein and ?-Lg content in whey samples changed from 0.07 to 0.8% and 0.24 to 0.29 g/L, respectively. Treatment with the use of pepsin enzyme, anion exchanges chromatography and ultrafiltration techniques, resulted to ?-Lg of 1.43, 6.56 and 43.59 folds respectively. Our results showed that ultrafiltration techniques are rapid and efficient that allows high protein yield and has advantages over other methods since it preserves the native structure of ?-Lg. Additionally, when the enzymatic hydrolysis was used together with ultrafiltration technique, it was found efficient and pure than the enzymatic hydrolysis together with dialyse membrane. Also this study concluded that pepsin enzyme treatment and anion exchange chromatography are economic methods but they are not efficient enough and very time consuming. However isolation efficiency can be increased the use of isolation methods together
dc.description.abstractThe aim of this study was to introduce a simple, high efficient and less expensive method for isolation of ?Lg from whey. Anion exchange chromatography, pepsin enzyme treatment and ultrafiltration tecniques were preferred for isolation process to compare differences. In addition to, centrifuge ultrafiltration techniques were using for the first time for isolation of ?-Lg from whey. Physicochemical analysis of whey samples indicated that protein and ?-Lg content in whey samples changed from 0.07 to 0.8% and 0.24 to 0.29 g/L, respectively. Treatment with the use of pepsin enzyme, anion exchanges chromatography and ultrafiltration techniques, resulted to ?-Lg of 1.43, 6.56 and 43.59 folds respectively. Our results showed that ultrafiltration techniques are rapid and efficient that allows high protein yield and has advantages over other methods since it preserves the native structure of ?-Lg. Additionally, when the enzymatic hydrolysis was used together with ultrafiltration technique, it was found efficient and pure than the enzymatic hydrolysis together with dialyse membrane. Also this study concluded that pepsin enzyme treatment and anion exchange chromatography are economic methods but they are not efficient enough and very time consuming. However isolation efficiency can be increased the use of isolation methods together
dc.identifier.endpage8
dc.identifier.issn2602-2834
dc.identifier.issue1
dc.identifier.startpage1
dc.identifier.trdizinid269285
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpZNU1qZzFOUT09
dc.identifier.urihttps://hdl.handle.net/11480/2357
dc.identifier.volume4
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.relation.ispartofFood and Health
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGıda Bilimi ve Teknolojisi
dc.titleCOMPARISON OF DIFFERENT METHODS FOR BETA LACTOGLOBULIN ISOLATION
dc.typeArticle

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