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    Antifungal Activity of Some Lactic Acid Bacteria Against Several Soilborne Fungal Pathogens Isolated from Strawberry Plants
    (2018) Canpolat, Elif; Doğaner, Müzeyyen Müge; Derviş, Sibel; Serçe, Çiğdem Ulubaş
    Developing as an alternative plant disease control method by using beneficialmicroorganisms and their metabolites has gained so much importance in recent years. Inthis study, the possibilities of using microorganisms which have potential antimicrobialeffects on controlling soil-borne fungi at strawberry production were investigated. Effectsof different lactic acid bacteria (LAB) strains on the development of several soil-bornefungi were studied in vitro and in vivo. LAB were screened for antifungal activity byusing cell free supernatant against Fusarium sp., Rhizoctonia sp., Macrophomina sp.,Botrytis sp., Phtopythium sp., Cylindrocarpon sp. and Pestalotiopsis sp. Cell freesupernatant of LAB isolates showed promising antifungal activity. In vitro effectivestrains of LAB were tested in pot experiments to search their effects on diseasedevelopment and plant growth. While the antifungal effects of all LAB strains tested invitro experiments exhibited promising results, in vivo experiments revealed similareffects on different fungi genera.
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    Bjerkandera adusta Collected from Niğde: Analysis of Total Phenolic Compound, Antioxidant, vnd Antimicrobial Properties
    (2022) Ildız, Elif; Canpolat, Şükrü; İşlek, Cemil; Canpolat, Elif; İşlek, Yasemin; Akata, Ilgaz
    Macrofungi have long been used as food and medicinal purposes by humankind. This study aimed to identify a macrofungus sample collected from Niğde, Türkiye in 2019 via molecular techniques and determine the content of total phenolic compounds, antioxidant and antimicrobial activities. According to data obtained from the sequence analysis of ITS fragment of rDNA the macrofungus sample was identified as Bjerkandera adusta (Willd.) P. Karst (1880). The sample was extracted with ethanol and methanol. Total phenolic compound content, antioxidant activity via DPPH scavenging method and antimicrobial activity via disc diffusion method of B. adusta was determined by using these extracts. The amount of total phenolic compounds was found as 772.28 ?g GAE/mL for a methanolic extract of B. adusta. The ethanol extract of B. adusta showed 79.66% scavenging activity of 0.1 mM DPPH solution. The highest inhibition zone diameter was measured as 28±1 mm against P. aeruginosa by ethanolic extract, while the lowest antimicrobial activity was found in 15 ?L methanol extract against S. typhimurium with an inhibition zone diameter of 8.7±1.2 mm.
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    Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA
    (2019) Canpolat, Elif; Süt, Burcu
    Microbial and chemical contamination is one of the biggest environmental problems since exposureto pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, areoften associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultativeanaerobic opportunistic bacterial pathogen, which is found in a variety of sources. In this study, weisolated and characterized P. lautus from Niğde Province, which is a wastewater disposal area andtherefore presents a great source of contamination. The bacterial isolate was grown, colonymorphology and Gram-reactions were observed. Bacteria were identified by 16S rDNA sequencinganalysis, which was performed using DNA samples isolated from pure cultures and amplified bypolymerase chain reaction (PCR). Sequencing results were compared against known bacterial strainsusing BLAST (Basic Local Alignment Search Tool) across databases. Antibiotic resistance against20 different antibiotics and heavy metal resistance against different concentrations of zinc, copper,iron, cobalt, chromium and silver containing compounds were tested via disk diffusion method.Inhibition zones were examined and minimum inhibitory concentrations were recorded. Our resultsindicated that P. lautus was resistant to a certain set of antibiotics, while it was sensitive againstothers. Similarly, the bacteria were resistant against high concentrations of most metals tested. Insummary, this study provided the first data regarding the isolation and characterization ofPaenibacillus lautus strain from a local source in Turkey, which will account as preliminary dataand guide our future efforts to fight against microbial contaminations.
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    Effect of molasses on hydrogen production by a new strain Rhodoplanes piscinae 51ATA
    (Pergamon-Elsevier Science Ltd, 2022) Canpolat, Elif; Ozturk, Ayten
    The production of hydrogen using microorganisms is an environment-friendly and less energy-intensive way of producing hydrogen. Rhodoplanes piscinae is a photosynthetic bacterium with the ability of hydrogen production under photosynthetic conditions. In this study, a new strain 51ATA was isolated from Lake Akkaya, Nigde, Turkey that is exposed to some industrial effluent charges. The new strain was identified as R. piscinae by phylogenetic analysis of the 16S ribosomal DNA (rDNA) sequence. The quality of molasses as a substrate for hydrogen production was evaluated by comparing it with other substrates, such as glucose and acetate. Five different culture media of various concentrations (1.0 g/L, 2.0 g/L, 5.0 g/L, 10 g/L, and 20 g/L) for each substrate were used. Results have shown that molasses was the best substrate for the biohydrogen production. The highest amount of biohydrogen obtained from each (20 g/L) substrate was (1.27 L H-2/L from molassescontaining culture), (0.72 L H-2/L from glucose-containing culture), and acetate-containing culture (0.21 L H-2/L) respectively. From these results, we could conclude that R.piscinae 51ATA strain is as good as the other bacterial species used for hydrogen production and may be considered as a high potential strain for hydrogen production when used in combination with molasses under phototrophic conditions. (C) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
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    Evaluation of Gold Nanoparticles in Terms of Their Use in Biomedical Applications
    (2019) Süt, Burcu; Canpolat, Elif
    Recently nanotechnology has become an integral part of modern biomedical applications.Accordingly, nanoparticles are considered as promising components for the development ofinnovative tags, probes, biosensors and carrier molecules for drug delivery. Spherical colloidal goldnanoparticles (AuNPs) are prime candidates to be utilized for these purposes due to their usefulphysical properties. However, in order for the gold nanoparticles to be used in nanomedicine, theirbiological properties should be extensively studied as well. Therefore, in this paper we chemicallysynthesized gold nanoparticles and studied their physical and biological characteristics to determinetheir potential use in medicine. Gold nanoparticles were synthesized by the reduction of chloroauricacid (HAuCl4) solution with sodium citrate. The physical properties of the AuNPs were determinedby UV–vis spectrophotometer and Zetasizer readings. The antimicrobial activity of the newlysynthesized gold nanoparticles on Escherichia coli, Salmonella infantis, Salmonella kentucky,Salmonella typhimurium, Salmonella enteritidis, Pseudomonas aeruginosa, Listeriamonocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis,Bacillus subtilis and Candida albicans were investigated via disk diffusion method. We found thatthe AuNPs were monodisperse, stable and not prone to aggregation with an average size of 22.12nm and an emission band at 522 nm. The disk diffusion tests revealed that the gold nanoparticles didnot have a significant growth inhibitory effect on the pathogens tested. In conclusion, here weshowed the successful synthesis of gold nanoparticles by a safe and non-toxic method. Furthermore,our evaluation of the antimicrobial activity of these nanoparticles suggests that these molecules couldbe considered as biologically safe molecules for future medical applications.
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    Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde
    (2021) Canpolat, Şükrü; Akata, Ilgaz; İşlek, Yasemin; Canpolat, Elif; İşlek, Cemil
    Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible\rAscomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the\rsamples were performed afterward. PCR products were sent for sequence analysis after ITS\rfragments were amplified with suitable primers concerning DNA isolation of samples. The obtained\rdata were compared with the Genbank database for samples’ determination. The mushroom samples\rwere determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were\rextracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion\rmethod. Antimicrobial zones against microorganisms were measured. Then DPPH radical\rscavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples\rwere studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta.\rWhen the DPPH radical scavenging activities are taken into account, the activity is increased\rdepending on the concentration. The highest DPPH scavenging activity was found in the extract of\rM. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts\rof mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 ?g GAE / mg extract.