Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Microbial and chemical contamination is one of the biggest environmental problems since exposureto pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, areoften associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultativeanaerobic opportunistic bacterial pathogen, which is found in a variety of sources. In this study, weisolated and characterized P. lautus from Niğde Province, which is a wastewater disposal area andtherefore presents a great source of contamination. The bacterial isolate was grown, colonymorphology and Gram-reactions were observed. Bacteria were identified by 16S rDNA sequencinganalysis, which was performed using DNA samples isolated from pure cultures and amplified bypolymerase chain reaction (PCR). Sequencing results were compared against known bacterial strainsusing BLAST (Basic Local Alignment Search Tool) across databases. Antibiotic resistance against20 different antibiotics and heavy metal resistance against different concentrations of zinc, copper,iron, cobalt, chromium and silver containing compounds were tested via disk diffusion method.Inhibition zones were examined and minimum inhibitory concentrations were recorded. Our resultsindicated that P. lautus was resistant to a certain set of antibiotics, while it was sensitive againstothers. Similarly, the bacteria were resistant against high concentrations of most metals tested. Insummary, this study provided the first data regarding the isolation and characterization ofPaenibacillus lautus strain from a local source in Turkey, which will account as preliminary dataand guide our future efforts to fight against microbial contaminations.
Açıklama
Anahtar Kelimeler
Biyoloji, Biyoteknoloji ve Uygulamalı Mikrobiyoloji, Çevre Bilimleri
Kaynak
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
9
