Determination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA

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dc.contributor.authorCanpolat, Elif
dc.contributor.authorSüt, Burcu
dc.date.accessioned2024-11-07T13:16:26Z
dc.date.available2024-11-07T13:16:26Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractMicrobial and chemical contamination is one of the biggest environmental problems since exposureto pathogenic microorganisms, such as food poisoning bacteria and their biological by-products, areoften associated with disease and allergic reactions. Paenibacillus lautus is an aerobic or facultativeanaerobic opportunistic bacterial pathogen, which is found in a variety of sources. In this study, weisolated and characterized P. lautus from Niğde Province, which is a wastewater disposal area andtherefore presents a great source of contamination. The bacterial isolate was grown, colonymorphology and Gram-reactions were observed. Bacteria were identified by 16S rDNA sequencinganalysis, which was performed using DNA samples isolated from pure cultures and amplified bypolymerase chain reaction (PCR). Sequencing results were compared against known bacterial strainsusing BLAST (Basic Local Alignment Search Tool) across databases. Antibiotic resistance against20 different antibiotics and heavy metal resistance against different concentrations of zinc, copper,iron, cobalt, chromium and silver containing compounds were tested via disk diffusion method.Inhibition zones were examined and minimum inhibitory concentrations were recorded. Our resultsindicated that P. lautus was resistant to a certain set of antibiotics, while it was sensitive againstothers. Similarly, the bacteria were resistant against high concentrations of most metals tested. Insummary, this study provided the first data regarding the isolation and characterization ofPaenibacillus lautus strain from a local source in Turkey, which will account as preliminary dataand guide our future efforts to fight against microbial contaminations.
dc.identifier.doi10.24925/turjaf.v7i9.1465-1468.2824
dc.identifier.endpage1468
dc.identifier.issn2148-127X
dc.identifier.issue9
dc.identifier.startpage1465
dc.identifier.trdizinid355041
dc.identifier.urihttps://doi.org/10.24925/turjaf.v7i9.1465-1468.2824
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/355041
dc.identifier.urihttps://hdl.handle.net/11480/12308
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241107
dc.subjectBiyoloji
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.subjectÇevre Bilimleri
dc.titleDetermination of Antibiotic and Heavy Metal Resistance in Paenibacillus lautus 51ATA
dc.typeArticle

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