The Anticancer Activity of Cetraria Islandica (L.) Ach in Breast Cancer Cells Through Crosstalk of Ampk-?1 and Erk1/2 Signalling
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In the present study, we aimed to evaluate the anticancer activities of Cetraria islandica(C.islandica) extracts on MCF-7 breast cancer cell lines. Cell viability, protein levels,apoptotic cells number, F-actin distribution were measured. Cell viability of MCF-7breast cancer cells was found to be reduced in a dose-dependent manner.EC50 values ofC.islandica on MCF-7 cells were found to be 9.2047 E-5 g/ml (cell amount) by usingintelligence system. Expressions of p53, caspase 3 and Bcl-2, were shown to be elevatedafter low doses of extract and diminished after high dose treatments. PPAR-? proteinlevel was decreased, although AMP-activated kinases-?1 (AMPK-?1) protein level wasincreasedin its extract groups. ERK1/2 protein level was also elevated in its extractgroups. 125 mg/ml of extract treated cells show a low decrease in actin filament density.MCF-7 cells with C.islandica treatment for 24 h increased the apoptotic cell percentage,though the cells-treated with C.islandica for 48 was high necrotic cells percentage.Consequently, the C.islandica extract treatment causes to elevate ERK1/2 and AMPK-?1protein levels, resulting in PPAR-? and then triggers the apoptosis by modulationcaspase-3 and P53 protein levels. Therefore, C.islandica might be a good candidate foranticancer tissue, especially soft tissue tumours.
Açıklama
Anahtar Kelimeler
Tıbbi Araştırmalar Deneysel, Onkoloji, Biyokimya ve Moleküler Biyoloji
Kaynak
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
6
Sayı
6
