Gümüş Nitrat (AgNO3) Çözeltisinin Capsicum annuum L. Hücre Süspansiyon Kültüründe Toplam Protein, Toplam Fenolik ve Bazı Antioksidant Enzim Aktiviteleri Üzerine Etkisi
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Ağır metal kirliliği, tarım ve insan sağlığı üzerinde olumsuz potansiyel etkileri olan önemli bir çevre sorunudur. Bu çalışmada, in vitro koşullarda çimlendirilmiş biber fidelerinin hipokotil eksplantlarından kallus elde edilmiş ve kalluslardan hücre süspansiyonları oluşturulmuştur. Biber hücre süspansiyon kültürlerine farklı konsantrasyonlarda ve zamanlarda ilave edilen gümüş nitrat (AgNO3) çözeltisinin, hücrelerin toplam protein ve toplam fenolik madde miktarları ile süperoksit dismutaz ve peroksidaz enzim aktiviteleri üzerine etkileri incelenmiştir. Toplam protein ve toplam fenolik madde miktarları, süperoksit dismutaz ve peroksidaz enzim aktiviteleri spektrofotometrik yöntemle belirlenmiştir. Gümüş metalinin biber bitkisi hücrelerinde toplam protein ve fenolik madde miktarını azalttığı, özellikle yüksek konsantrasyonlarda ilk 24 saatlik süre içerisinde antioksidan enzim aktivitelerini arttırdığı saptanmıştır.
Heavy metal pollution is a significant environmental problem with negative potential impacts on agriculture and human health. In this study, calluses were obtained by using in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from these calluses. The effect of silver nitrate (AgNO3) solution added in different concentrations and times to cell suspension cultures of pepper on total protein and total phenolic compound amounts, superoxide dismutase and peroxidase enzyme activities were analysed. Total protein and total phenolic amounts, superoxide dismutase and peroxidase enzyme activities were detected by spectrophotometric methods. When the effects of silver metal on pepper plant were examined, it was determined that silver metal reduced the total protein and phenolic content in the pepper plant cells and especially at higher concentration, in the first 24 hour period, antioxidant enzyme activities increased.
Heavy metal pollution is a significant environmental problem with negative potential impacts on agriculture and human health. In this study, calluses were obtained by using in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from these calluses. The effect of silver nitrate (AgNO3) solution added in different concentrations and times to cell suspension cultures of pepper on total protein and total phenolic compound amounts, superoxide dismutase and peroxidase enzyme activities were analysed. Total protein and total phenolic amounts, superoxide dismutase and peroxidase enzyme activities were detected by spectrophotometric methods. When the effects of silver metal on pepper plant were examined, it was determined that silver metal reduced the total protein and phenolic content in the pepper plant cells and especially at higher concentration, in the first 24 hour period, antioxidant enzyme activities increased.
Açıklama
Anahtar Kelimeler
Gıda Bilimi ve Teknolojisi, Ziraat, Ortak Disiplinler
Kaynak
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
5
Sayı
12
