Effects of different genotypes and gamma ray doses on haploidization using irradiated pollen technique in squash
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Fourteen genotypes and 3 different gamma ray doses were tested to develop an efficient haploidization protocol in squash. For this purpose, male flowers collected 1 day before anthesis were irradiated with 150, 200, and 300 Gy gamma ray doses, and female flowers were pollinated with the irradiated pollens the next day. In the first year of the study, 1858 embryos were obtained from 219 fruits. While 1358 of these were found in fruits irradiated with a 150 Gy gamma dose, the remaining 500 embryos were found in fruits irradiated with 200 Gy. From fruits irradiated with 150 and 200 Gy gamma doses, 9.12 and 3.53 haploid embryos per 100 seeds were obtained, respectively. While Genotype 3 was the most successful genotype with 12.42 embryos per 100 seeds, the minimum embryo numbers were obtained from Genotype 4 with 1.46 embryos per 100 seeds. In the second experimental year, 8 genotypes and the same gamma doses were used, and 2625 haploid and 1378 diploid embryos were obtained from 217 fruits. At irradiation doses of 150, 200, and 300 Gy, 2010, 539, and 76 haploid embryos were found, respectively. Genotype 6 was the most successful genotype with 13.35 embryos per 100 seeds.
Açıklama
Anahtar Kelimeler
Cucurbita pepo, haploid embryo, irradiation dose
Kaynak
TURKISH JOURNAL OF BIOLOGY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
38
Sayı
3
