Effects of different genotypes and gamma ray doses on haploidization using irradiated pollen technique in squash

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dc.contributor.authorBaktemur, Gokhan
dc.contributor.authorYucel, Namik Kemal
dc.contributor.authorTaskin, Hatira
dc.contributor.authorComlekcioglu, Songul
dc.contributor.authorBuyukalaca, Saadet
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2014
dc.departmentNiğde ÖHÜ
dc.description.abstractFourteen genotypes and 3 different gamma ray doses were tested to develop an efficient haploidization protocol in squash. For this purpose, male flowers collected 1 day before anthesis were irradiated with 150, 200, and 300 Gy gamma ray doses, and female flowers were pollinated with the irradiated pollens the next day. In the first year of the study, 1858 embryos were obtained from 219 fruits. While 1358 of these were found in fruits irradiated with a 150 Gy gamma dose, the remaining 500 embryos were found in fruits irradiated with 200 Gy. From fruits irradiated with 150 and 200 Gy gamma doses, 9.12 and 3.53 haploid embryos per 100 seeds were obtained, respectively. While Genotype 3 was the most successful genotype with 12.42 embryos per 100 seeds, the minimum embryo numbers were obtained from Genotype 4 with 1.46 embryos per 100 seeds. In the second experimental year, 8 genotypes and the same gamma doses were used, and 2625 haploid and 1378 diploid embryos were obtained from 217 fruits. At irradiation doses of 150, 200, and 300 Gy, 2010, 539, and 76 haploid embryos were found, respectively. Genotype 6 was the most successful genotype with 13.35 embryos per 100 seeds.
dc.description.sponsorshipRijk Zwaan Company
dc.description.sponsorshipThe authors would like to thank the Rijk Zwaan Company for supporting this study. The authors are also grateful to Mr Necmettin Kurtul for his valuable contributions to and recommendations for the study during the editing process.
dc.identifier.doi10.3906/biy-1309-5
dc.identifier.endpage327
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84898461559
dc.identifier.scopusqualityQ2
dc.identifier.startpage318
dc.identifier.trdizinid162987
dc.identifier.urihttps://dx.doi.org/10.3906/biy-1309-5
dc.identifier.urihttps://hdl.handle.net/11480/4273
dc.identifier.volume38
dc.identifier.wosWOS:000334166200002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCucurbita pepo
dc.subjecthaploid embryo
dc.subjectirradiation dose
dc.titleEffects of different genotypes and gamma ray doses on haploidization using irradiated pollen technique in squash
dc.typeArticle

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