The effect of boric acid on copine-7 expression and bioactivity in dental pulp stem cells
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Energy Nuclear and Mining Research Institute
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Boron has significant impact on the mineral composition of teeth. Copine-7 (Cpne-7) is secreted by pre-ameloblasts and induces dentin formation via differentiation of mesenchymal cells of dental. The goal of this study was to see how boric acid affected the bioactivity and expression of Cpne-7, collagen type I in dental pulp stem cell. The expression of Cpne-7, COL-I were assessed in the boric acid treated pulp cells by molecular method on 3 and 8 days exposure. When comparing the different boric acid concentrations to the control group in a proliferation experiment, no significant differences were noted. At 10 ng/mL boric acid, a rise in the number of mineralized nodules was observed. Boric acid concentrations (1 ng/mL, 10 ng/mL) increased the transcripts of Cpne-7 on day 3 and 8. Additionally, 1 ng/ mL boric acid concentration significantly upregulated of Cpne-7 expression compared to control group on day 3 and 8. When the 1-10 ng boric acid was compared to control group COL-I expression remarkably enhanced in cell. According to the current findings that boric acid may be a potent regulator of Cpne-7, which is a promising candidate for new dentin formation in regenerative dentistry, in dental pulp stem cells and provide osteogenic efficacy in therapies aimed at dentin formation. © 2022, Turkish Energy Nuclear and Mining Research Institute. All rights reserved.
Açıklama
Anahtar Kelimeler
Boric acid, Cpne-7, Pulp mesenchymal stem cells
Kaynak
Journal of Boron
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
7
Sayı
2
