Verification of enzymatic ethanol analysis method and method comparison with headspace gas chromatography
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Walter De Gruyter Gmbh
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives This study aimed to verify the enzymatic ethanol analysis method and compare the technique with headspace gas chromatography (HS/GC). Materials and methods The limit of the blank, limit of detection, and limit of quantification, precision, linearity, and accuracy of the enzymatic process were evaluated based on the data obtained. In addition, a method comparison was made between the enzymatic method and the HS/GC analysis method. Results It was seen that the enzymatic method was linear in the range of 14-450 mg/dL, the coefficient of variation and bias values ranged between 0.5-1.09% and 4.52-9.78%, respectively. The quantitative accuracy was 2.13% and 2.23% at concentrations of 41.16 and 95.82 mg/dL, respectively. When the regression analysis was performed between the results obtained using the enzymatic alcohol dehydrogenase (ADH) and HS/GC methods, y=0.966x + 2.084 linear relationship formula and regression coefficient of r(2)=0.9987, it was found that it showed a good agreement at a confidence interval of 5%. Conclusions The findings showed that the enzymatic method had good linearity, excellent precision, and a high correlation with the higher-order comparator method, the HS/GC method, in the ethanol analysis.
Açıklama
Anahtar Kelimeler
enzymatic method, ethanol, headspace gas chromatography, method comparison, method verification, enzimatik metot, etanol, headspace gaz kromatografisi, metot karsilastirma, metot verifikasyonu
Kaynak
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
47
Sayı
5
