Verification of enzymatic ethanol analysis method and method comparison with headspace gas chromatography

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dc.authoridUcar, Fatma/0000-0001-7354-375X
dc.authoridOZTURK, Alpaslan/0000-0003-4525-3477
dc.authoridYay, Fatih/0000-0002-1872-8716
dc.authoridYalcindag, Ali/0000-0003-1846-9248
dc.contributor.authorOzturk, Alpaslan
dc.contributor.authorTemel, Ismail
dc.contributor.authorYalcindag, Ali
dc.contributor.authorUcar, Fatma
dc.contributor.authorYay, Fatih
dc.date.accessioned2024-11-07T13:31:20Z
dc.date.available2024-11-07T13:31:20Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjectives This study aimed to verify the enzymatic ethanol analysis method and compare the technique with headspace gas chromatography (HS/GC). Materials and methods The limit of the blank, limit of detection, and limit of quantification, precision, linearity, and accuracy of the enzymatic process were evaluated based on the data obtained. In addition, a method comparison was made between the enzymatic method and the HS/GC analysis method. Results It was seen that the enzymatic method was linear in the range of 14-450 mg/dL, the coefficient of variation and bias values ranged between 0.5-1.09% and 4.52-9.78%, respectively. The quantitative accuracy was 2.13% and 2.23% at concentrations of 41.16 and 95.82 mg/dL, respectively. When the regression analysis was performed between the results obtained using the enzymatic alcohol dehydrogenase (ADH) and HS/GC methods, y=0.966x + 2.084 linear relationship formula and regression coefficient of r(2)=0.9987, it was found that it showed a good agreement at a confidence interval of 5%. Conclusions The findings showed that the enzymatic method had good linearity, excellent precision, and a high correlation with the higher-order comparator method, the HS/GC method, in the ethanol analysis.
dc.identifier.doi10.1515/tjb-2021-0280
dc.identifier.endpage563
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85130710997
dc.identifier.scopusqualityQ4
dc.identifier.startpage557
dc.identifier.trdizinid1167847
dc.identifier.urihttps://doi.org/10.1515/tjb-2021-0280
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1167847
dc.identifier.urihttps://hdl.handle.net/11480/14763
dc.identifier.volume47
dc.identifier.wosWOS:000793427700001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectenzymatic method
dc.subjectethanol
dc.subjectheadspace gas chromatography
dc.subjectmethod comparison
dc.subjectmethod verification
dc.subjectenzimatik metot
dc.subjectetanol
dc.subjectheadspace gaz kromatografisi
dc.subjectmetot karsilastirma
dc.subjectmetot verifikasyonu
dc.titleVerification of enzymatic ethanol analysis method and method comparison with headspace gas chromatography
dc.typeArticle

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