Isolation of Anti-Escherichia coli O157:H7 Bacteriophages and Determination of Their Host Ranges
Küçük Resim Yok
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to isolate, purify and determine host ranges of bacteriophagesinfecting Escherichia coli O157:H7 from different environment such as river/streamwater, sewage, raw food, animal troughs, wastewaters of food processing plants,slaughterhouse and fish farms. For screening of E. coli O157:H7 bacteriophages, 92samples were used. It was found that in respect to anti-E. coli O157:H7 bacteriophages,food processing wastewaters, sewage and slaughterhouse wastewaters are the richestsources, and streams, troughs and fisheries wastewater are rich in the middle, and rawfoods were the poorest source. A total of 37 phages were isolated and purified. Thephages counts of the purified samples were changed among 30×103 - 34×108 PFU/mL.The isolated phages were generally infective against E. coli O157:H7 and E. coli strainsand 81.08% of the phages (30 out of 37) formed clear plaques and were capable to lyse atleast 1 out of 5 E. coli O157:H7 strains. In addition to E. coli, some phages were capableto infect some Salmonella enterica serovars. This results show that inhibitor spectra ofthe phages were wide.
Açıklama
Anahtar Kelimeler
Mikrobiyoloji, Biyoteknoloji ve Uygulamalı Mikrobiyoloji, Viroloji
Kaynak
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
6
Sayı
9
