Isolation of Anti-Escherichia coli O157:H7 Bacteriophages and Determination of Their Host Ranges

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dc.contributor.authorYıldırım, Zeliha
dc.contributor.authorSakin, Tuba
dc.contributor.authorÇoban, Fatma
dc.date.accessioned2024-11-07T13:16:25Z
dc.date.available2024-11-07T13:16:25Z
dc.date.issued2018
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe aim of this study was to isolate, purify and determine host ranges of bacteriophagesinfecting Escherichia coli O157:H7 from different environment such as river/streamwater, sewage, raw food, animal troughs, wastewaters of food processing plants,slaughterhouse and fish farms. For screening of E. coli O157:H7 bacteriophages, 92samples were used. It was found that in respect to anti-E. coli O157:H7 bacteriophages,food processing wastewaters, sewage and slaughterhouse wastewaters are the richestsources, and streams, troughs and fisheries wastewater are rich in the middle, and rawfoods were the poorest source. A total of 37 phages were isolated and purified. Thephages counts of the purified samples were changed among 30×103 - 34×108 PFU/mL.The isolated phages were generally infective against E. coli O157:H7 and E. coli strainsand 81.08% of the phages (30 out of 37) formed clear plaques and were capable to lyse atleast 1 out of 5 E. coli O157:H7 strains. In addition to E. coli, some phages were capableto infect some Salmonella enterica serovars. This results show that inhibitor spectra ofthe phages were wide.
dc.identifier.doi10.24925/turjaf.v6i9.1200-1208.2000
dc.identifier.endpage1208
dc.identifier.issn2148-127X
dc.identifier.issue9
dc.identifier.startpage1200
dc.identifier.trdizinid329173
dc.identifier.urihttps://doi.org/10.24925/turjaf.v6i9.1200-1208.2000
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/329173
dc.identifier.urihttps://hdl.handle.net/11480/12284
dc.identifier.volume6
dc.indekslendigikaynakTR-Dizin
dc.language.isotr
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241107
dc.subjectMikrobiyoloji
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.subjectViroloji
dc.titleIsolation of Anti-Escherichia coli O157:H7 Bacteriophages and Determination of Their Host Ranges
dc.typeArticle

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