Frequency of thiopurine S-methyltransferase gene variations in turkish children with acute leukemia

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dc.contributor.authorAkın D.F.
dc.contributor.authorAşlar-öner D.
dc.contributor.authorKürekçi E.
dc.contributor.authorAkar N.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractIn this study we aim to determine the genotype distribution and allele frequencies of common TPMT (*2, *3A, *3B and *3C) polymorphisms in Turkish children with acute leukemia. The study population consisted of 169 patients aged between 1 and 15 years who were admitted to Losante Pediatric Hematology and Children’s Hospital with the diagnosis of acute leukemia. Genotyping of TPMT polymorphisms was screened with real-time PCR using fluorescence melting curve detection analysis. We found that the frequencies of four allelic variants of TPMT are *2 (238 G>C) (0,0%), *3A (460G>A and 719A>G) (1.7%), *3B (460G>A) (1,7%) and *3C (719A>G) (2.4%). Frequency of TPMT alleles increases the efficacy of leukemia treatment. Thus, TPMT genotyping can be useful for optimizing 6-MP therapy. © 2018, Turkish Journal of Pediatrics. All rights reserved.
dc.description.sponsorshipAnkara Universitesi
dc.description.sponsorshipthe World Medical Association (Declaration of Helsinki) for experiments involving humans. The Ankara University, School of Medicine Ethics Committee approved the study protocol (Project No.14-646-14/2014) and informed consent was provided by the patients’ parents. Blood samples were collected with EDTA-containing tubes and DNA was extracted from peripheral blood and bone marrow leukocytes with MagNA Pure automatic DNA isolation instrument (Roche Diagnostics, Manheim, Germany).
dc.identifier.doi10.24953/turkjped.2018.02.005
dc.identifier.endpage152
dc.identifier.issn0041-4301
dc.identifier.issue2
dc.identifier.pmid30325120
dc.identifier.scopus2-s2.0-85054890408
dc.identifier.scopusqualityQ3
dc.identifier.startpage147
dc.identifier.trdizinid351666
dc.identifier.urihttps://dx.doi.org/10.24953/turkjped.2018.02.005
dc.identifier.urihttps://hdl.handle.net/11480/1741
dc.identifier.volume60
dc.identifier.wosWOS:000447379800005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTurkish Journal of Pediatrics
dc.relation.ispartofTurkish Journal of Pediatrics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChildhood
dc.subjectLeukemia
dc.subjectPolymorphism
dc.subjectTPMT
dc.titleFrequency of thiopurine S-methyltransferase gene variations in turkish children with acute leukemia
dc.typeArticle

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