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Öğe Antioxidant and Antigenotoxic Potential of Infundibulicybe geotropa Mushroom Collected from Northwestern Turkey(Hindawi Ltd, 2020) Sevindik, Mustafa; Akgul, Hasan; Selamoglu, Zeliha; Braidy, NadyInfundibulicybe geotropa (Bull.) Harmaja is an edible mushroom found in Bolu province in northwestern Turkey. The chemical composition and bioactivity of these mushrooms has not been previously investigated. We examined the phenolic composition, elemental content, and antioxidant and antigenotoxic effects of methanol extracts of fruiting bodies. The phenolic compounds in the fungal samples were determined using high-performance liquid chromatography (HPLC), and element content was determined using atomic absorption spectrophotometry. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined using the commercially available Rel assay kit. The antigenotoxic effects of the extract were determined using the MTT assay to assess cell viability and the alkaline single-cell gel electrophoresis assay (Comet assay). The total phenolic content (ppm) of I. geotropa was found to be catechin (361 +/- 2.31), clorogenic acid (553.54 +/- 5.06), and coumaric acid (9.93 +/- 0.25). The TAS, TOS, and OSI of the extract were 1.854 +/- 0.051 mmol/L, 30.385 +/- 0.399 mu mol/L, and 1.639 +/- 0.067, respectively. The elemental levels were within normal range. In HT22 mouse hippocampal neuronal cells, the extract (100 and 200 mu g/ml) showed no genotoxic potential and ameliorated hydrogen peroxide- (H2O2-) induced oxidative DNA damage. I. geotropa may be considered a good nutrient due to its phenolic constituents and antioxidant potential.Öğe Antioxidant, antimicrobial and neuroprotective effects of Octaviania asterosperma in vitro(Taylor & Francis Ltd, 2021) Sevindik, Mustafa; Akgul, Hasan; Selamoglu, Zeliha; Braidy, NadyOctaviania asterosperma (hypogeous Basidiomycota) We investigated the phenolic composition, and antioxidant, antimicrobial and antigenotoxic effects of methanol extracts of fruiting bodies from Octaviania asterosperma. The total phenolic content (ppm) of O. asterosperma was found to be catechin (54.73 +/- 4.68), epicatechin (123.90 +/- 8.52), caffeic acid (4.23 +/- 0.97), p-hydroxybenzoic acid (37.72 +/- 3.84), cinnamic acid (58.07 +/- 5.40), gallic acid (56.64 +/- 6.39), clorogenic acid (80.76 +/- 4.92) and coumaric acid (2.45 +/- 0.15). The total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were 3.410 +/- 0.099 mmol/L, 7.548 +/- 0.147 mu mol/L and 0.221 +/- 0.005 respectively. O. asterosperma showed some promising antimicrobial activity. The extract showed no genotoxic potential and attenuated hydrogen peroxide (H2O2)-induced oxidative DNA damage in neurons. Pre-treatment with O. asterosperma maintained mitochondrial function, reduced expression levels of cleaved-caspase-3 and apoptosis-inducing factor (AIF) when HT22 cells were exposed to pathophysiological concentrations of GLU (25 mM) and modulated protein kinase B (Akt), the mammalian target of rapamycin (mTOR), and the phosphotase and tensin homolog on chromosome ten (PTEN). O. asterosperma is an important food for the treatment or management of neurodegenerative disorders due to its phenolic content and potent antioxidant and anti-excitotoxic effects.Öğe Biochemical changes induced by grape seed extract and low level laser therapy administration during intraoral wound healing in rat liver: an experimental and in silico study(Taylor & Francis Inc, 2018) Erdemli, Mehmet Erman; Salmas, Ramin Ekhteiari; Durdagi, Serdar; Akgul, Hasan; Demirkol, Mehmet; Aksungur, Zeynep; Selamoglu, ZelihaIn the present study, the changes that occur in rat liver tissue as a result of the use of grape seed extract (GSE) and low level laser therapy (LLLT) in intraoral wound (IW) healing are analyzed using biochemical parameters. Diode laser application groups received 8J/cm(2) dose LLLT once a day for 4days (810nm wavelength, continuous mode, 0.25W, 9s). As a result of the biological parameter analysis, it was determined that the oxidative damage caused by the IWs and recovery period on 7th and 14th days could be substantially removed with GSE applications that have antioxidant capacity especially in rat liver tissue. In addition, the active compound of grape seed, catechin is studied in the active site of glycogen synthase kinase 3 (GSK3) target using molecular modeling approaches. Post-processing molecular dynamics (MD) results for catechin is compared with a standard GSK3 inhibitor. MD simulations assisted for better understanding of inhibition mechanism and the crucial amino acids contributing in the ligand binding. These results along with a through free energy analysis of ligands using sophisticated simulations methods are quite striking and it suggests a greater future role for simulation in deciphering complex patterns of molecular mechanism in combination with methods for understanding drug-receptor interactions.Öğe Biological Activities of Wild Poisonous Mushroom Entoloma sinuatum (Bull.) P. Kumm (Boletales)(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Bal, Celal; Baba, Hayri; Akata, Ilgaz; Sevindik, Mustafa; Selamoglu, Zeliha; Akgul, HasanIn many studies conducted in recent years, it has been found that the beneficial natural ingredients found in mushrooms are very important in curing various diseases and eliminating disease factors. It has been reported that fungi that spread in different countries have biological activities. In this study, antioxidant level (TAS). oxidant level (TOS), and antibacterial and antifungalactivity of Entoloma sinuatum (Bull.) P. Kumm were determined. Ethanol extract of the mushroom was extracted in a Soxhlet apparatus. Antioxidant and oxidant levels were determined using Rel Assay TAS and TOS kits. Also. antimicrobial activity was tested against bacteria l and fungal strains using the agar dilution method. As a result of the studies. the TAS value of the mushroom was determined 2.64 +/- 0.15. the TOS value vas 6.58 +/- 0.23, and the OSI (Oxidative stress index) value was 0.25 +/- 0.02. In addition, it was determined that the ethanol extract of the mushroom was effective against bacteria at 200 and 400 mu g/mL, and against fungi at 50 mu g/mL concentrations. As a result, it was determined that E. sinuatum is a natural antioxidant and antimicrobial agent.Öğe DETERMINATION OF ANTIOXIDANT, ANTIMICROBIAL, DNA PROTECTIVE ACTIVITY AND HEAVY METALS CONTENT OF LAETIPORUS SULPHUREUS(Parlar Scientific Publications (P S P), 2018) Sevindik, Mustafa; Akgul, Hasan; Dogan, Muhittin; Akata, Ilgaz; Selamoglu, ZelihaSince ancient times, people consumed mushrooms as nutrients, especially during the rainy season. The present study aimed to determine the therapeutic potential of an edible mushroom, Laetiporus sulphureus (Bull.) Murrill. Thus, its antioxidant activity was determined by DPPH method and antimicrobial activity was determined with modified agar dilution method. TAS (total antioxidant status), TOS (total oxidant status) and OSI (oxidative stress index) values were determined using Rel Assay kits. PBR 322 supercoiled DNA was used to identify the DNA protective activity. Heavy metal content was determined with atomic absorption spectrophotometry using the wet decomposition method. In conclusion, it was determined that L. sulphureus demonstrated high biological activity and it was considered that the mushroom can be used as a natural resource in alternative medicine.Öğe DETERMINATION OF THERAPEUTIC POTENTIAL OF MENTHA LONGIFOLIA SSP LONGIFOLIA(Parlar Scientific Publications (P S P), 2017) Sevindik, Mustafa; Akgul, Hasan; Pehlivan, Mustafa; Selamoglu, ZelihaThe present study aimed to determine antioxidant, antimicrobial activities and oxidative stress properties of the ethanol extracts of Mentha longifolia L. Hudson subsp. longifolia. Antioxidant activity was determined with the DPPH method. Antimicrobial activity was tested on six different microorganisms (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida tropicalis) using modified agar dilution method. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were determined using Rel Assay kits. It was observed that DPPH and antimicrobial potentials of plant samples varied based on the locations they were collected. As a result, it was considered that plant samples could be used as natural antioxidant and antimicrobial resources, but suitable regions for OSI values should be selected.Öğe Determination of therapeutic potential of mentha longifolia ssp. Longifolia(Parlar Scientific Publications, 2017) Sevindik, Mustafa; Akgul, Hasan; Pehlivan, Mustafa; Selamoglu, ZelihaThe present study aimed to determine antioxidant, antimicrobial activities and oxidative stress properties of the ethanol extracts of Mentha longifolia L. Hudson subsp. longifolia. Antioxidant activity was determined with the DPPH method. Antimicrobial activity was tested on six different microorganisms (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida tropicalis) using modified agar dilution method. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were determined using Rel Assay kits. It was observed that DPPH and antimicrobial potentials of plant samples varied based on the locations they were collected. As a result, it was considered that plant samples could be used as natural antioxidant and antimicrobial resources, but suitable regions for OSI values should be selected. © by PSP.Öğe DETERMINING THE TOTAL ANTIOXIDANT STATUS AND OXIDATIVE STRESS INDEXES OF HONEY SAMPLES OBTAINED FROM DIFFERENT PHYTOGEOGRAPHICAL REGIONS IN TURKEY(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2015) Akyol, Ethem; Selamoglu, Zeliha; Dogan, Hamide; Akgul, Hasan; Unalan, AdnanThis study aimed to determine the total antioxidant and oxidant status and oxidative stress index of honey samples which were collected from 11 different phytogeographical regions in Turkey. Honey samples were kept at 4 degrees C until analysis. Total antioxidant status, total oxidant status and oxidative stress indexes were defined with in vitro analyses in extracted honey samples, and then, the obtained data were compared statistically. These characteristics of honey were analysed by the most sensitive and reliable measurement kits developed newly. In addition, biological activities of honey samples originating from different regions were also compared. As a result of the study, the highest total antioxidant capacity was observed (P<0.01) in the honey samples obtained from Duzce and Mugla regions. The results also showed that total antioxidant capacities of honey samples could change according to various phytogegraphic properties of different regions in Turkey. Besides, antioxidant properties of honey depended on phenolic compounds that could change according to plant vegetation of honey obtained from different regions.Öğe Determining the total antioxidant status and oxidative stress indexes of honey samples obtained from different phytogeographical regions in Turkey(Parlar Scientific Publications, 2015) Akyol, Ethem; Selamoglu, Zeliha; Dogan, Hamide; Akgul, Hasan; Unalan, AdnanThis study aimed to determine the total antioxidant and oxidant status and oxidative stress index of honey samples which were collected from 11 different phytogeographical regions in Turkey. Honey samples were kept at 4 °C until analysis. Total antioxidant status, total oxidant status and oxidative stress indexes were defined with in vitro analyses in extracted honey samples, and then, the obtained data were compared statistically. These characteristics of honey were analysed by the most sensitive and reliable measurement kits developed newly. In addition, biological activities of honey samples originating from different regions were also compared. As a result of the study, the highest total antioxidant capacity was observed (PO.Ol) in the honey samples obtained from Duzce and Mugla regions. The results also showed that total antioxidant capacities of honey samples could change according to various phytoge- ographic properties of different regions in Turkey. Besides, antioxidant properties of honey depended on phenolic compounds that could change according to plant vegetation of honey obtained from different regions.Öğe ENVIRONMENTAL EFFECTS ON BIOLOGIC ACTIVITIES OF POLLEN SAMPLES OBTAINED FROM DIFFERENT PHYTOGEOGRAPHICAL REGIONS IN TURKEY(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2016) Selamoglu, Zeliha; Akgul, Hasan; Dogan, HamideBee pollen is considered a health food with a wide range of therapeutic properties. This study aimed to determine the environmental effects on biologic activities of pollen samples obtained from eleven different environmental sources and geographical origins in Turkey (Artvin, Balikesir, Duzce, Edirne, Kahramanmaras, Mersin, Mugla, Nigde, Ordu, Sivas and Van). Pollen samples were kept at +4 degrees C until extracted. Total antioxidant status, total oxidant status and oxidative stress indexes in pollen samples extracted with specific methods were defined with in vitro analyses and results comparised. These characteristics of pollen were analysed by the most sensitive and reliable measurement kits developed newly. The highest total antioxidant capacity was observed (P<0.01) in pollen samples obtained from Artvin and Balikesir provinces. Total antioxidant capacities of pollen samples collected from different regions demonstrated differences because of different phytogeographic characteristics of regions in Turkey. Antioxidant properties of pollen depended on phenolic compounds that could change according to plant vegetation of pollen obtained from different regions.Öğe In-vitro Antioxidant Activities of the Ethanolic Extracts of Some Contained-Allantoin Plants(Shaheed Beheshti Univ, Sch Pharmacy, 2017) Selamoglu, Zeliha; Dusgun, Cihan; Akgul, Hasan; Gulhan, Mehmet FuatIt has been investigated the in-vitro antioxidant properties of ethanol extracts of the containedallantoin plants in this study. Contained-allantoined plant samples Plantago lanceolata, Plantago major, Robinia pseudoacacia, Platanus orientalis and Aesculus hippocastanum were tested at different concentrations. The antioxidant activities of plant samples were analysed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method, cupric reducing antioxidant capacity (CUPRAC), reducing power assay and beta-carotene bleaching method. Plantago major plant showed the highest antioxidant capacity compared to other plant extracts in results of the in-vitro assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method with 90.25 %, cupric reducing antioxidant capacity (CUPRAC) with 1.789 %, reducing power assay (FRAP) with 1.321 % and beta-carotene bleaching method with 78.01 % in 1 mg/mL. The lowest antioxidant activity was determined in Robinia pseudoacacia plant. In conclusion, allantoin shows antioxidant properties and it has the positive effect on total antioxidant capacity.Öğe OXIDATIVE STRESS INDEX AND ANTIOXIDANT CAPACITY OF LEPISTA NUDA COLLECTED FROM GAZİANTEP/TURKEY(Yildiz Technical University, 2019) Bal, Celal; Sevındık, Mustafa; Akgul, Hasan; Selamoglu, ZelihaThe objective of the present study was to determine antioxidant capacity and oxidative stress index of Lepista nuda (Bull.) Cooke mushrooms collected in Şahinbey (Gaziantep/Turkey) using novel research kits that could conduct high sensitive and reliable measurements with in vitro analysis methods. Its antioxidant activity was determined by DPPH method and TAS (total antioxidant status), TOS (total oxidant status) and OSI (oxidative stress index) values were determined using Rel Assay kits. Study findings determined that the TAS value was 3.102 mmol/L, the TOS value was 36.920 ?mol/L and the OSI value was 1.190 for the mushroom. It was observed that the radical scavenging activity of DPPH increased in higher concentrations. It was concluded that L. nuda samples collected in regions with low TOS values could be used as better antioxidant sources. © 2019 by the Author(s).Öğe OXIDATIVE STRESS INDEX AND ANTIOXIDANT CAPACITY OF LEPISTA NUDA COLLECTED FROM GAZIANTEP/TURKEY(Yildiz Technical Univ, 2019) Bal, Celal; Sevindik, Mustafa; Akgul, Hasan; Selamoglu, ZelihaThe objective of the present study was to determine antioxidant capacity and oxidative stress index of Lepista nuda (Bull.) Cooke mushrooms collected in Sahinbey (Gaziantep/Turkey) using novel research kits that could conduct high sensitive and reliable measurements with in vitro analysis methods. Its antioxidant activity was determined by DPPH method and TAS (total antioxidant status), TOS (total oxidant status) and OSI (oxidative stress index) values were determined using Rel Assay kits. Study findings determined that the TAS value was 3.102 mmol/L, the TOS value was 36.920 mu mol/L and the OSI value was 1.190 for the mushroom. It was observed that the radical scavenging activity of DPPH increased in higher concentrations. It was concluded that L. nuda samples collected in regions with low TOS values could be used as better antioxidant sources.Öğe Phenolic composition, total antioxidant, antiradical and antimicrobial potential of endemic Glaucium Alakirensis(Yildiz Technical Univ, 2024) Ozcandir, Aysen; Mohammed, Falah Saleh; Sevindik, Mustafa; Aykurt, Candan; Selamoglu, Zeliha; Akgul, HasanPlants have been used by many communities for different purposes such as food, shelter, medicine. The present study aimed to determine phenolic content and biological potential of Glaucium alakirensis Aykurt, K. Yildiz & A. 0zcandir, which is endemic to Antalya (Turkey). Phenolic composition of the plant was analyzed with HPLC device, while antiradical activity was determined by DPPH method. Antimicrobial tests were conducted with agar dilution method against standard bacteria and fungus strains. Total antioxidant and oxidant status were determined using Rel Assay kits. As a result of HPLC analysis, the presence of catechin, chlorogenic acid, hydroxybenzoic acid, quercetin and gallic acid was determined. Plant extracts were effective against microorganisms at concentrations of 50-200 mu g/mL. It was determined that the plant has high antiradical activity. It is also thought to be used as a natural source for relieving oxidative stress.Öğe Phenolic Contents, Oxidant/Antioxidant Potential and Heavy Metal Levels in Cyclocybe cylindracea(Assoc Pharmaceutical Teachers India, 2018) Sevindik, Mustafa; Akgul, Hasan; Bal, Celal; Selamoglu, ZelihaIntroduction: Mushroom species have been used for medical purposes and as nutrients since the old times. The present study aimed to determine phenolic content, antioxidant activity and heavy metal content of Cyclocybe cylindracea (DC.) Vizzini & Angelini mushroom. Materials and Methods: Phenolic contents were screened with HPLC devices. TAS, TOS and OSI values were determined using Rel Assay kits. Heavy metal content were determined with atomic absorption method using an atomic absorption spectrophotometer. Result and Discussion: Gallic acid, hesperidin, catechin, syringic acid and hydroxybenzoic acid were detected in the mushroom. It was determined that the heavy metal content in C. cylindracea were generally at optimal levels and the TOS value of mushroom was high. Conclusion: C. cylindracea could be consumed as a good antioxidant source as long as it is obtained from safe areas.Öğe The Effects on Oxidative Systems in Liver Tissues of Systemic Ozone Application after Critical Size Hone Defect Surgery in Rat Mandibles(Ars Docendi, 2019) Erdemli, Mehmet Erman; Akgul, Hasan; Selamoglu, ZelihaExamination of the changes that non-toxic doses of antioxidant action ozone used in increasing osteogenic activity by dentists causes on rat liver tissues. The effects of ozone applications that are significant in mandibular bone defect therapies and oral and maxillofacial surgery practice on oxidant / antioxidant system in rat liver tissues are investigated by analyzing malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) parameters. While there were statistically significant reductions in MDA levels and SOD activities after 15 and 45 days long ozone applications on critical mandibular bone defect induced rat livers, statistically significant increases were observed in GSH levels and CAT activities (P <= 0.05). This is the first study where the effects of ozone therapies used in treatment of mandibular bone defect on liver tissue oxidant / antioxidant system in rats are scrutinized with the analysis of biochemical parameters. It was determined that ozone applications did not have any negative effects on liver tissue oxidant / antioxidant system and concurrently had positive effects on liver antioxidant system and to reveal the biochemical mechanism completely, further studies are needed.
